期刊
ELECTROANALYSIS
卷 21, 期 23, 页码 2575-2583出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200900243
关键词
Metallothionein; Differential pulse voltammetry; ELISA; Chicken yolk antibodies; Human blood serum; Immunoassays; Antibodies
资金
- INCHEMBIOL [MSM 0021622412, MSM 6215712402, MSM 0021620813, GAAV IAA401990701]
In this Study, two methods for metallothioneins (MT) determination in a biological sample were compared. Particularly, twenty five human and nine pig blood serum samples and liver and kidney samples from thirty five carps (Cyprinus carpio) were analyzed by enzyme-linked immunosorbent assay and differential pulse voltammetry Brdicka's reaction. The results obtained by these two methods were in good agreement. For commercially available NIT standard the correlation coefficient between the single concentrations signal height was 0.99. In biological samples the correlation coefficients were 0.90 for fish liver and kidney samples, 0.91 for pig blood serum and 0.93 for human blood serum.
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