Prussian Blue Modified Carbon Ionic Liquid Electrode: Electrochemical Characterization and Its Application for Hydrogen Peroxide and Glucose Measurements

Prussian blue modified carbon ionic liquid electrodes (PB-CILEs) were fabricated using chemical and electrochemical procedures. Chemically fabricated PB-CILE exhibited an excellent sensitivity (0.0866 mu A mu M(-1)), low detection limit (0.01 mu M) and two linear ranges (0.01-1 and 1-600 mu M) toward hydrogen peroxide. Then, glucose oxidase (GOx) was immobilized on the surface of PB-CILE to fabricate glucose biosensor using three different procedures involving cross linking with glutaraldehyde (GLU) and bovine serum albumin (BSA), entrapment into the Nafion matrix and covering with a sol-gel layer. Glucose biosensor fabricated using cross linking procedure showed the best sensitivity (0.0019 mu A mu M(-1)) and operational stability for glucose.