Autooxidative activity of chlorogenic acid and damage to DNA

The interaction of chlorogenic acid with double-stranded calf thymus DNA was investigated in solution by cyclic voltammetry at a glassy carbon electrode (GCE). The different working electrodes were prepared by covering solution containing different components onto the surface of GCE. The autooxidative activity of chlorogenic acid and inducement of DNA damage were evaluated by employing the prepared working electrode in pH 5.0, 0.10 M acetate buffer solution with differential pulse voltammetry (DPV). The influences of temperature and metal ions (cupric ions) on the extent of DNA damage were examined. Chlorogenic acid possessed autooxidative activity and could Induce DNA damage under certain conditions. The increasing temperature and existing cupric ions could enhance the autooxidative capablilty of chlorogenic acid and enlarge the extent of DNA damage. It was possible that the DNA damage induced by chlorogenic acid preferentially took place at guanosine-containing segments, with the formation of 8-oxo-deoxyguanosine (a biomarker of DNA oxidative damage). A mechanism on the autooxidative activity of chlorogenic acid and inducing DNA damage was proposed.