期刊
ELECTROANALYSIS
卷 20, 期 18, 页码 2040-2046出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200804287
关键词
horseradish peroxidase; o-aminophenol; screen-printed carbon electrode; electrochemical detection.; point-of-care
资金
- National Institutes of Health CounterACT Program [NS058161-01]
- National Institute of Environmental Health Sciences (NIEHS), NIH [U54 ES16015]
- DOE's Office of Biological and Environmental Research
- National University of Singapore Postgraduate Research Scholarship
- PNNL
A rapid, simple and sensitive electrochemical assay of horseradish peroxidase (HRP) performed on disposable screen-printed carbon electrode was developed. HRP activities were monitored by square-wave voltammetric (SWV) measuring the electroactive enzymatic product in the presence of o-aminophenol and hydrogen peroxide substrate solution. SWV analysis demonstrated a greater sensitivity and shorter analysis time than the widely used amperometric and differential-pulsed voltammetric methods. The voltammetric characteristics of substrate and enzymatic product as well as the parameters of SWV analysis were optimized. Under optimized conditions. a linear response for HRP from 0.003 to 0.1 U/mL and a detection limit of 0.002 U/mL (1.25 x 10(-15) mol in 25 mu L) were obtained with a good precision (RSD = 8%; n = 6). This rapid and sensitive HRP assay with microliter-assay volume could be readily integrated to portable devices and point-of-care (POC) diagnosis applications.
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