期刊
ELECTROANALYSIS
卷 20, 期 2, 页码 115-122出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200704040
关键词
carbon nanotubes; proteins; immunosensor; polylysine
资金
- NIEHS NIH HHS [R01 ES013557, R01 ES013557-02] Funding Source: Medline
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R01ES013557] Funding Source: NIH RePORTER
Amperometric immunosensor configurations featuring covalently bound anti-biotin antibodies (Ab) embedded into a polylysine (PI,L)-single walled carbon nanotube (SWCNT) composite layer were evaluated. Assemblies were made by first oxidizing pyrolytic graphite (PG) electrodes to form surface carboxylic acid groups, to which PLL, SWCNTs and anti-biotin were covalently linked. Incorporating SWCNT into PLL-antibody assemblies improved the amperometric detection limit for biotin (Ag) labeled with horseradish peroxidase to 10 fmol mL(-1). Anti-biotin embedded into the PLL matrix had improved thermal stability and retained its binding ability for biotin after exposure to temperatures of 42 degrees C for up to 3 hours, while the noncrosslinked antibody was inactivated at this temperature in several minutes.
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