期刊
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
卷 6, 期 5, 页码 860-867出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpclett.5b00034
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资金
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences [DE-FG02-07ER15892]
- Dutch organization for scientific research (NWO-ALW) via a Vici grant
- Netherlands Organization for Sciences, Council of Chemical Sciences (NWO-CW) via a TOP grant [700.58.305]
- European Research Council [267333]
- EU FP7 project PAPETS [GA 323901]
- Royal Netherlands Academy of Arts and Sciences (KNAW)
- University of Pretoria's Research Development Programme [A0W679]
- Center for Molecular Analysis and Design at Stanford University
- Kenneth and Nina Tai Stanford Graduate Fellowship
- EMBO
In photosynthetic light harvesting, absorbed sunlight is converted to electron flow with near-unity quantum efficiency under low light conditions. Under high light conditions, plants avoid damage to their molecular machinery by activating a set of photoprotective mechanisms to harmlessly dissipate excess energy as heat. To investigate these mechanisms, we study the primary antenna complex in green plants, light-harvesting complex II (LHCII), at the single-complex level. We use a single-molecule technique, the Anti-Brownian Electrokinetic trap, which enables simultaneous measurements of fluorescence intensity, lifetime, and spectra in solution. With this approach, including the first measurements of fluorescence lifetime on single LHCII complexes, we access the intrinsic conformational dynamics. In addition to an unquenched state, we identify two partially quenched states of LHCII. Our results suggest that there are at least two distinct quenching sites with different molecular compositions, meaning multiple dissipative pathways in LHCII. Furthermore, one of the quenched conformations significantly increases in relative population under environmental conditions mimicking high light.
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