期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 100, 期 -, 页码 122-130出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2013.10.009
关键词
Bacillus thuringiensis; Metal detoxification; NanoLC/FT-ICR; Oxidative stress; Silver nanopartide; Two dimensional electrophoresis
资金
- Research Council of Shahid Beheshti University
- Iran Nanotechnology Initiative Council
Emerging technologies in functional genomics and proteomics provide a way of achieving high-throughput analyses, understanding effects on protein populations and sub-populations and follow up environmental stresses. To accomplish these, the action of homemade spherical Silver nanoparticles colloidal suspension (AgNPs) against Bacillus thuringiensis (isolate from Oryza sativa L rhizosphere) was investigated by a proteomic approach (2-DE and NanoLC/FT-ICR MS identification). Thirty four responsive (up/down regulated) proteins were identified. Proteomic results revealed that an exposure of B. thuringiensis cells with different concentrations of AgNPs resulted in an accumulation of envelope protein precursors, indicative of the dissipation of a proton motive force. Identified proteins are involved in oxidative stress tolerance, metal detoxification, transcription and elongation processes, protein degradation, cytoskeleton remodeling and cell division. The expression pattern of these proteins and their possible involvement in the nontoxicity mechanisms were discussed. (C) 2013 Elsevier Inc. All rights reserved.
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