期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 93, 期 -, 页码 39-44出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2013.03.038
关键词
Oxidative stress; Immune response; Phthalate esters; Fish embryo
资金
- Ministry of Education [20113227110020]
- State key laboratory of Environmental Chemistry and Ecotoxicology [KF2011-20]
In the present study, we analyzed the oxidative stress related indices and immune related gene expression of zebrafish embryos after a short-term exposure to various concentrations of di-n-butyl phthalate (DBP), diethyl phthalate (DEP) and their mixture (DBP-DEP) from 4 h post-fertilization (hpf) to 96 hpf. Exposure to the chemicals was found to enhance the production of reactive oxygen species (ROS) and lipid peroxidation (LPO) in a concentration-dependent manner. Simultaneously, adaptive responses to DBP/DEP-induced oxidative stress were observed. The activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were all increased in a concentration-dependent manner. The transcription of innate immune related genes including interferon gamma (IFN gamma), interleukin-1 beta (1L1 beta), Myxovirus resistance (Mx), tumor necrosis factor a (TNF alpha), CC-chemokine, CXCL-clc, lysozyme (Lyz) and complement factor C3B (C3) were up-regulated upon DBP, DEP and their mixture exposure, suggesting the induction of immune response. In addition, co-exposure to DBP-DEP also induced antioxidant defense and immune response in zebrafish embryo. The results demonstrat that DBP/DEP exposure could induce the antioxidant and immune responses in zebrafish embryos. (C) 2013 Elsevier Inc. All rights reserved.
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