Effects of Pb stress on nutrient uptake and secondary metabolism in submerged macrophyte Vallisneria natans

For better understanding the metabolic adaptations to Pb stress in submerged plants, the alterations in mineral elements uptake and in secondary metabolism were studied in leaves of Vallisneria natans (Lour.) Hara exposed to 0-100 mu M Pb for 0-7 d. Pb content increased in leaves in a dose-dependent way. The increase of calcium, magnesium and iron content and the decrease of phosphorus, potassium and manganese content were detected in leaves of V. natans under Pb stress, while no significant changes were detected in copper and zinc concentration. Meanwhile, there was an increase in the concentrations of total phenolic and flavonoids. Pb treatment caused an increase in the catalytic activities of shikimate dehydrogenase, phenylalanine ammonialyase and polyphenol oxidase. The results suggest that nutrient uptake and secondary metabolism were actively regulated by V. natans plants in response to Pb stress. (C) 2011 Elsevier Inc. All rights reserved.