Interrelationships between cadmium, zinc and antioxidants in the liver of the rat exposed orally to relatively high doses of cadmium and zinc

Zinc (Zn) reduces cadmium (Cd)-induced toxicity in the liver although it increases Cd tissue burden in some conditions; hence, the present study is designed to study the relationships between Cd, Zn and antioxidants in the liver of rats exposed to Cd. Livers of male rats which received orally relatively high doses of Cd (200 mg Cd/L as Cd chloride or Cd (200 mg/L)+Zn (500 mg Zn as Zn chloride) during five weeks, were investigated. Cd induced an accumulation of Cd and Zn in parallel to depletion in important variables (GSH, GSH/GSSG, CuZn-SOD and GPx activities) and to elevation in others (Cd/Zn and GSSG). Cd, did not affect CuZn SOD/GPx, nor Mn-SOD in the liver. Cd accumulation, Cd/Zn, CuZn SOD activity and CuZn SOD/GPx, was increased remarkably under Zn action. Zn supply ameliorated GSH level and partially reversed the decrease in GSH/GSSG, but it did not ameliorate GPx activity. Analysis showed high correlations between Cd and the majority of the variables, while Zn was positively correlated with only GSSG. We suggest from our results that Zn has indirect ameliorative effects on Cd-induced toxicity in the liver and that the increase in Cd retention is probably the key mechanism modulating, in the case of relatively high doses of Cd, the antioxidant response during exposure to Cd and Zn. (C) 2011 Elsevier Inc. All rights reserved.