期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 73, 期 4, 页码 491-499出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2010.01.018
关键词
Larval growth; Sea-urchin; Embryo; Larva; Bioassay; Method
资金
- Xunta de Galicia (Galician Government)
- Spanish Ministerio de Ciencia y Tecnologia [CTM2006-13880-C03-01/MAR]
The sea-urchin embryo test (SET) has been frequently used as a rapid, sensitive, and cost-effective biological tool for marine monitoring worldwide, but the selection of a sensitive, objective, and automatically readable endpoint, a stricter quality control to guarantee optimum handling and biological material, and the identification of confounding factors that interfere with the response have hampered its widespread routine use. Size increase in a minimum of n=30 individuals per replicate, either normal larvae or earlier developmental stages, was preferred to observer-dependent, discontinuous responses as test endpoint. Control size increase after 48 h incubation at 20 degrees C must meet an acceptability criterion of 218 mu m. In order to avoid false positives minimums of 32 parts per thousand salinity, 7 pH and 2 mg/L oxygen, and a maximum of 40 mu g/L NH3 (NOEC) are required in the incubation media. For in situ testing size increase rates must be corrected on a degree-day basis using 12 degrees C as the developmental threshold. (C) 2010 Elsevier Inc. All rights reserved.
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