期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 72, 期 7, 页码 1899-1904出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2009.04.016
关键词
Bensulfuron-methyl; SOD; CAT; ATPase; Oxidative stress; Native PAGE; Isoenzyme
资金
- National Hi-Tech Research and Development Program [2007AA06Z329]
- Science and Technology Projects of Zhejiang Province [2007C23036, 2008C13014-3]
- International Cooperation Project in Science and Technology of Zhejiang Province [2008C14038]
Oxidative stress enzymes, superoxide dismutase (SOD), catalase (CAT), and ATRase, from two Gram-positive bacteria and one Gram-negative bacterium, respectively, were tested for response to the oxidative stress caused by bensulfuron-methyl (EISM). Native PAGE was used to detect the SOD isoenzyme profiles of these bacteria. All three bacteria possessed a basal level of SOD, CAT, and ATPase activity prior to being exposed to BSM. Enzyme activities changed in a BSM-concentration-dependent manner after exposure to BSM for 24 h. Activity of all the enzymes was increased and reached the first activity peak after being exposed to BSM at 1 or 1.5 h, respectively, then a decline occurred, and after that another simulation appeared at 9 h or 14 h. Only one and three detectable SOD isoenzyme bands were observed in Gram-positive strains and the Gram-negative strain, respectively. BSM could bring short-term ecotoxicity to three bacteria, but the effect of BSM was not lethal. (C) 2009 Elsevier Inc. All rights reserved.
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