期刊
ECOTOXICOLOGY
卷 19, 期 4, 页码 796-803出版社
SPRINGER
DOI: 10.1007/s10646-009-0456-5
关键词
Microcystin; Malus pumila; Tissue culture; ELISA; Superoxide dismutase; Peroxidase
资金
- National 863 High Science and Technology Project of China [AA-64-10-30]
- Major Science and Technology Specific Project of Zhejiang Province [2008C12009]
The bioaccumulation and harmful effects of microcystins (MCs) and the activity of peroxidase (POD) and superoxide dismutase (SOD) were examined in the apple (Malus pumila) exposed in vitro with the crude extract of toxic cyanobacterial blooms from Dianchi Lake in southwestern China. The results showed that the growth and proliferation of M. pumila shoots in vitro decreased markedly after exposure to microcystins above 0.3 mu g/ml. Recovered microcystins determined by enzyme-linked immunosorbent assay (ELISA) in M. pumila shoot cultures increased with exposure time and concentration. After 14 days exposure to the concentration of 3 mu g/ml microcystins, M. pumila shoot cultures accumulated microcystins up to a concentration of 510.23 +/- A 141.10 ng MC-LR equiv/g FW (fresh weight), equivalent to an accumulation rate of 36.45 ng/g day. POD activity was significantly increased after 7 days exposure to 3 mu g/ml microcystins. After 14 days of exposure, microcystins caused POD to increase significantly at the concentration of 0.3 and 3 mu g/ml. The activity of SOD was not affected by microcystins at concentrations up to 3 mu g/ml on 7 days. After 14 days exposure to microcystins, SOD activity increased significantly at the concentration of 0.3 and 3 mu g/ml in M. pumila shoot cultures.
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