期刊
ECOLOGY
卷 90, 期 12, 页码 3526-3535出版社
WILEY
DOI: 10.1890/08-1695.1
关键词
Alaska, USA; compound-specific stable isotope analysis; delta C-13; essential amino acids; eukaryotes; gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS); prokaryotes
类别
资金
- NSF [IOB 05-52015, DEB-0620579]
- Bonanza Creek LTER
- Div Of Biological Infrastructure
- Direct For Biological Sciences [0821856] Funding Source: National Science Foundation
Amino acids play an important role in ecology as essential nutrients for animals and as currencies in symbiotic associations. Here we present a new approach to tracing the origins of amino acids by identifying unique patterns of carbon isotope signatures generated by amino acid synthesis in plants, fungi, and bacteria (C-13 fingerprints''). We measured amino acid delta C-13 from 10 C-3 plants, 13 fungi, and 10 bacteria collected and isolated from a boreal forest in interior Alaska, USA, using gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Microorganisms were cultured under amino-acid-free conditions and identified based on DNA sequences. Bacteria, fungi, and plants generated consistent, unique C-13 fingerprints based on the more complex amino acids (five or more biosynthetic steps) that are classified as essential for animals. Linear discriminant analysis classified all samples correctly with >99% certainty and correctly classified nearly all insect samples from a previous study by diet. Our results suggest that delta C-13 fingerprints of amino acids could provide a powerful in situ assay of the biosynthetic sources of amino acids and a potential new tool for understanding nutritional linkages in food webs.
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