期刊
ACS NANO
卷 9, 期 6, 页码 6511-6521出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b02357
关键词
quantum dot; polyethyleneimine; cellular labeling; targeting; gene delivery; oxygen probe; amphiphilic encapsulation
类别
资金
- National Research Foundation (NRF) - Korean government (MEST) [2011-0019635]
- National R&D Program for Cancer Control, Ministry for Health and Welfare, Republic of Korea [1320220]
- Engineering Research Center of the National Research Foundation (NRF) [2011-0030075]
- Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI12C1642]
- NRF - Ministry of Science, ICT and Future Planning (MSIP) of Korea [NRF-2014K1A3A1A49070433]
- National Research Foundation of Korea [2011-0019635] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Amphiphilic polyethyleneimine derivatives (amPEIs) were synthesized and used to encapsulate dozens of quantum dots (QDs). The QD-amPEI composite was similar to 100 nm in hydrodynamic diameter and had the slightly positive outer surface that suited well for cellular internalization. The QD-amPEI showed very efficient QD cellular labeling with the labeled cell fluorescence intensity more than 10 times higher than conventional techniques such as Lipofectamine-assisted QD delivery. QD-amPEI was optimal for maximal intracellular QD delivery by the large QD payload and the rapid endocytosis kinetics. QD-amPEI platform technology was demonstrated for gene delivery, cell-specific labeling, and ratiometric oxygen sensing. Our QD amPEI platform has two partitions: positive outer surface and hydrophobic inside pocket. The outer positive surface was further exploited for gene delivery and targeting. Co-delivery of QDs and GFP silencing RNAs was successfully demonstrated by assembling siRNAs to the outer surfaces, which showed the transfection efficiency an order of magnitude higher than conventional gene transfections. Hyaluronic acids were tethered onto the QD amPEI for cell-specific targeted labeling which showed the specific-to-nonspecific signal ratio over 100. The inside hydrophobic compartment was further applied for cohosting oxygen sensing phosphorescence Ru dyes along with QDs. The QD-Ru-amPEI oxygen probe showed accurate and reversible oxygen sensing capability by the ratiometric photoluminescence signals, which was successfully applied to cellular and spheroid models.
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