期刊
ECOLOGICAL ENGINEERING
卷 71, 期 -, 页码 126-132出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ecoleng.2014.07.025
关键词
Wastewater treatment; Saline wastewater; Microbial communities; Submerged fixed bed bioreactor; 454 Pyrosequencing
资金
- Project of Excellence - Junta de Andalucia [P06-RNM-01533]
- WIMEK Graduate School
- Netherlands Genomics Initiative (ECOLINC)
The influence of salt (NaCl) on bacterial and archaeal communities in a submerged fixed bed bioreactor system for the treatment of urban wastewater was determined by DGGE and 454 pyrosequencing of PCR-amplified 16S ribosomal RNA gene fragments. Cluster analysis of DGGE fingerprints showed significant differences in the community structure dependent on the salt concentration in the influent. Proteobacteria was found to be the dominant bacterial phylum in all experiments, with alpha-Proteobacteria being the main order at low salinity and gamma-Proteobacteria the dominant order at high salinity. Euryarchaeota was the main archaeal phylum in all experiments, with all microorganisms corresponding to methanogenic archaea. Whereas bacterial alpha-diversity decreased as salinity increased, archaeal alpha-diversity increased with higher NaCl concentrations. (C) 2014 Elsevier B.V. All rights reserved.
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