期刊
ACS NANO
卷 9, 期 6, 页码 5846-5856出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b00430
关键词
atomic force microscopy; force mapping; force spectroscopy; cell elasticity; cell mechanics; micropatterns; cell adhesion
类别
资金
- ANR [ANR-11-LABX-0054, ANR-11-IDEX-0001-02]
- European Research Council (ERC) [310080]
In multicellular organisms, cell shape and organization are dictated by cell cell or cell extracellular matrix adhesion interactions. Adhesion complexes crosstalk with the cytoskeleton enabling cells to sense their mechanical environment. Unfortunately, most of cell biology studies, and cell mechanics studies in particular, are conducted on cultured cells adhering to a hard, homogeneous, and unconstrained substrate with nonspecific adhesion sites, thus far from physiological and reproducible conditions. Here, we grew cells on three different fibronectin patterns with identical overall dimensions but different geometries (del, T, and Y), and investigated their topography and mechanics by atomic force microscopy (AFM). The obtained mechanical maps were reproducible for cells grown on patterns of the same geometry, revealing pattern-specific subcellular differences. We found that local Young's moduli variations are related to the cell adhesion geometry. Additionally, we detected local changes of cell mechanical properties induced by cytoskeletal drugs. We thus provide a method to quantitatively and systematically investigate cell mechanics and their variations, and present further evidence for a tight relation between cell adhesion and mechanics.
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