期刊
ACS NANO
卷 10, 期 1, 页码 257-264出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b03959
关键词
AFM; high-bandwidth; multifrequency; harmonic; cells
类别
资金
- Stanford Bio-X Interdisciplinary Initiatives Partnership
- Center for Probing the Nanoscale, an NSF NSEC [PHY 0830228]
- NIH/NIAID [K08 AI079268]
- NIH/NIGMS [R01 GM110482]
- NSF [CBET 1264833]
- Stanford Child Health Research Institute
The cytoskeleton controls cellular morphology and mediates the mechanical interactions between a cell and its environment. Atonic force Microscopy (AFM) has the unique capability to map cytoskeletal mechanics and structures with nanometer resolution. However, whole-cell cytomechanical imaging with conventional AFM techniques is limited by low imaging speed. Here, we present fast nanomechanical mapping of cells using high-bandwidth AFM (HB-AFM), where >10(6) nanoindentation measurements were acquired in similar to 10 min-a task that would take weeks to finish using conventional AFM. High-bandwidth measurements enabled capture of the entire tip-sample interaction for each tap on cells, engendering a new measurement (force phase) that exceeds the contrast of conventional tapping mode and enabling spectral visualization of >10 harmonics. The abundance of measurements allowed discovery of subtle cytomechanical features, including the stiffness of fibers of the cellular spectrin network in situ. This approach bridges HB-AFM and high-harmonic imaging and opens future opportunities for measuring the dynamic mechanical properties of living cells.
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