期刊
DYES AND PIGMENTS
卷 99, 期 3, 页码 537-542出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2013.06.031
关键词
Two-photon fluorescence microscopy; Fluorescent probes; Hydrogen sulfide; Thiolysis; High selectivity; Cells imaging
资金
- National Key Project for Basic Research of China [2009CB724706]
- Chinese Academy of Sciences
- State Key Laboratory of Fine Chemicals of China [KF1105]
Fluorescent probes for hydrogen sulfide have received considerable attention because of the biological significance of H2S recognized recently. Two-photo microscopy offers advantages of increased penetration depth, localized excitation, and prolonged observation time. However, two-photon fluorescent probes for H2S are still rare. In this work, we introduced a dinitrophenyl ether group into the 4-position of 1,8-naphthalimide, which acts as the H2S reactive site, to efficiently yield compound NI-NHS as a two-photo fluorescent probe for H2S. The probe NI-NHS has a high selectivity for H2S over competitive anions and sulfide-containing analytes. This probe exhibits turn-on fluorescence detection of H2S in bovine serum and two-photon fluorescent imaging of H2S in living cells. (C) 2013 Elsevier Ltd. All rights reserved.
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