期刊
DYES AND PIGMENTS
卷 84, 期 1, 页码 121-127出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2009.07.004
关键词
Cyanine dye; Fluorescent hybrid silica nanoparticles; Optical imaging; Neuronal survival; Internalization
资金
- Nanomat-ASP [Docup 2000-2006]
- Fondazione San Paolo
Spherical silica nanoparticles containing fluorescent trimethine indocyanine dyes (lambda(abs) = 547 nm, lambda(em) = 570 nm) were prepared using a water-in-oil microemulsion method. The nanoparticles were of similar to 50 nm diameter and were almost monodispersed in aqueous solution at pH 5.5. Entrapment of dye molecules in the silica matrix stabilised photoemission over several hours of continuous irradiation. The photoemission intensity of the indocyanine was increased 13-fold over that recorded in solution. As each nanoparticle contained similar to 110 dye molecules, the photoemission brightness of each particle was enhanced by three orders of magnitude. The fluorescent nanoparticles have been tested as imaging tools in in vitro tests. As an example of non-macrophagic cells, a highly differentiated neuronal cell line (GT1-7) was used and the results showed that the prepared nanoparticles can be incorporated into these cells with no apparent toxicity for up to three days. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据