期刊
DRUG TESTING AND ANALYSIS
卷 4, 期 6, 页码 460-467出版社
WILEY
DOI: 10.1002/dta.378
关键词
prednisolone; LC-MS; MS; WADA; glucocorticosteroids
资金
- Ministry of Youth Affairs & Sports, Government of India
The use of prednisolone and prednisone is prohibited by the World Anti-Doping Agency (WADA) due to their performance-enhancing effect. The purpose of the present work was to explore the possibility of identification and detection of various metabolites of prednisolone by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in excretion study samples. Ten metabolites of prednisolone could be identified namely prednisone (11-oxo metabolite) [M-1], 6-beta-OH-prednisolone [M-2], 20-beta-OH-prednisolone [M-3], 20-a-OH-prednisolone [M-4], 20-a-OH-prednisone [M-5], 20-beta-OH-prednisone [M-6], 2 tetrahydro epimers of 20-beta-OH-prednisolone [M-7], 2 tetrahydro epimers of 20-a-OH-prednisolone [M-8], 2 tetrahydro epimers of 20-gamma-OH-prednisone [M-9], and 2 tetrahydro epimers of 20-aOH-prednisone [M-10]. Prednisolone was administered in 10-, 20-, and 40-mg dosage to healthy volunteers to study detection of various metabolites. The parent, M-1, M-2, and M-3 could be detected up to 72?h while rest of the metabolites were detectable up to 24?h after drug administration. The detection of newer metabolites of the drug can further be used for confirmatory purposes. Copyright (c) 2012 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据