Voltammetric determination of glutathione in haemolysed erythrocyte and tablet samples using modified-multiwall carbon nanotubes paste electrode

A chemically modified electrode was prepared by incorporating p-aminophenol into multiwall carbon nanotubes paste matrix. Cyclic voltammetry, square wave voltammetry, double potential step chronoamperometry, and electrochemical impedance spectroscopy were used to investigate the electrochemical behaviour of glutathione at the chemically modified electrode prepared. According to the results, p-aminophenol multiwall carbon nanotubes paste electrode (p-APMWCNTPE) showed high electrocatalytic activity for glutathione oxidation, producing a sharp oxidation peak current at about +0.285 vs Ag/AgCl reference electrode at pH 5.0. Chronoamperometry was also used to determine glutathione's catalytic rate constant and diffusion coefficient at p-APMWCNTPE. The square wave voltammetric peak current of glutathione increased linearly with glutathione concentration in the range of 2.0?x?107 1.0?x?104?mol?L1 with a detection limit of 9.0?x?108?mol?L1. The method was also successfully employed as a selective, simple, and precise method for the determination of glutathione in haemolysed erythrocyte, tablet, and urine samples. Copyright (c) 2011 John Wiley & Sons, Ltd.