期刊
DRUG DELIVERY
卷 20, 期 8, 页码 338-348出版社
INFORMA HEALTHCARE
DOI: 10.3109/10717544.2013.834412
关键词
Behenic acid; camptothecin; Compritol 888; ATO; solid lipid nanoparticles; supercritical; fluid technology
Camptothecin (CPT) and its analogs exhibit remarkable anti-tumor activity, due to their ability to inhibit DNA topoisomerase I. However, its use is limited by the lack of solubility and stability of the active lactone form. An attractive alternative is the encapsulation of CPT within liposomes. In this study, CPT was incorporated into solid lipid nanoparticles (SLN) based on the triglyceride, Compritol 888 ATO, using supercritical fluid technology without requiring the use of harmful solvents. This drug delivery system was characterized and its cytotoxicity effect was evaluated by measuring MCF7 and MCF10A cell viability as a function of drug loading during a 48-h treatment. Results showed that after 10 h of treatment, MCF7 cells displayed an IC50 of 0.23 +/- 0.034 mM at a 1:5 (CPT: SLN) loading and 0.22 +/- 0.027 mM at a 1: 10 loading, whereas MCF10A cells displayed an IC50 of 0.40 +/- 0.036 mM at 1: 5 and 0.60 +/- 0.063 mM at 1: 10. On the other hand, the IC50 of free CPT was 0.57 +/- 0.035 mM and 1.07 +/- 0.077 mM for MCF7 and MCF10A cells, respectively. Cellular uptake and retention measurements in both cells displayed a two-fold increase when using the SLN formulation. The results from this study showed that the cytotoxic effects of CPT in a SLN formulation improved when compared with those seen with free CPT. The results of this study showed that delivery of CPT as a SLN formulation could be a promising strategy for enhancing its chemotherapeutic effects.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据