期刊
DRUG AND CHEMICAL TOXICOLOGY
卷 33, 期 4, 页码 421-429出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/01480541003739229
关键词
RVS glycoprotein (36 kDa); bisphenol A; interleukin-4; interleukin-10
资金
- Biotechnology Research Institute of Chonnam National University
Bisphenol A (BPA) is one of the estrogen mimic environmental hormones and a chemical used for the wrapping foods, toy products for children, biomedical equipment, and machines. It can exert toxic effects, such as occurring allergy-related diseases. This study demonstrates that glycoprotein isolated from Rhus verniciflua Stokes (RVS glycoprotein) has an inhibitory activity of T-helper type 2 (Th2) cytokines [ Interleukin (IL)-4 and -10]. First, it was shown that RVS glycoprotein inhibits the proliferation of lymphocytes and scavenges intracellular reactive oxygen species (ROS). Then, the activities of mitogen-activated protein kinase (MAPK), GATA-binding protein-3 (GATA-3), t-box expressed in T-cells (T-bet), and Th2 cell-related cytokine (IL-4 and -10) were evaluated in BPA (50 mu M)-stimulated primary cultured mouse lymphocytes, using immunoblot analysis and reverse-transcription polymerase chain reaction (RT-PCR). The results showed that the RVS glycoprotein (50 mu g/mL) inhibited the proliferation of lymphocytes, intracellular ROS, and activity of p38 MAPK dose dependently. In the transcriptional factors for the oriented differentiation of T-helper cells, the RVS glycoprotein (50 mu g/mL) significantly suppressed the GATA-3, whereas it enhanced T-bet. Also, the RVS glycoprotein (100 mu g/mL) significantly attenuated Th2-related cytokines (IL-4 and -10). Taken together, the results obtained from this study suggest that the RVS glycoprotein may help in preventing allergy-related immune dysfunction, such as that produced by BPA.
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