期刊
DOMESTIC ANIMAL ENDOCRINOLOGY
卷 41, 期 2, 页码 74-80出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.domaniend.2011.04.002
关键词
Leptin receptor isforms; Crucian carp; Hypoxia; Fasting
资金
- Department of Education of Zhejiang Province, China [Y200702196]
To understand leptin signaling pathway in the crucian carp (Carassius carassius). we cloned 3 leptin receptor isoform complementary DNAs (ie, the long form [cclpr-L], the short form [cclpr-s1]. and the secreted form [cclpr-s2]). Variant cclpr-L had a 3,255-bp open reading frame and a complete intracellular domain with box 1 and box 2 consensus sequences. By contrast, cclpr-s1 contained only 4 amino acids in its intracellular domain, without the box 1 motif, which is conserved among membrane-bound leptin receptor short isoforms in mammals. Variant cclpr-s2 had no transmembrane domain, suggesting that it is a soluble form of the receptor, and alternative splicing of cclpr-s2 mRNA employs a different mechanism for the generation of soluble leptin receptor by intron retention. The fasting-treated fish showed significantly lower cclpr-L mRNA levels in gill tissue than the control group, whereas cclpr-s2 mRNA levels did not vary significantly among the groups. Treatment with hypoxia significantly increased mRNA levels of both cclpr-L and cclpr-s2 in gill tissue. To our knowledge, this is the first study of leptin receptor isoforms expression in teleosts. (C) 2011 Elsevier Inc. All rights reserved.
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