期刊
DNA RESEARCH
卷 22, 期 1, 页码 101-107出版社
OXFORD UNIV PRESS
DOI: 10.1093/dnares/dsu043
关键词
E. coli; O-antigen biosynthesis gene cluster; horizontal gene transfer; O serogroup; genomic diversity
资金
- Health Labor Sciences Research Grants from the Ministry of Health, Labor, and Welfare, Japan [H25-Syokuhin-Wakate-018, H24-Shinkou-Ippan-012]
- Adaptable and Seamless Technology Transfer Program through Target-driven R&D from Japan Science and Technology Agency [AS242Z00217P]
- Scientific Research Grant on Priority Areas from the University of Miyazaki
- Program to Disseminate Tenure Tracking System from the Japanese Ministry of Education, Culture, Sports, Science, and Technology
- Wellcome Trust grant [098051]
- University of Miyazaki, Japan
- Grants-in-Aid for Scientific Research [24780044, 25460542] Funding Source: KAKEN
The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria. The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity. Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups. By comparing these sequences, we identified 161 well-defined O-AGCs. Based on the wzx/wzy or wzm/wzt gene sequences, in addition to 145 singletons, 37 serogroups were placed into 16 groups. Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs. Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized. These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
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