期刊
DNA RESEARCH
卷 19, 期 2, 页码 117-130出版社
OXFORD UNIV PRESS
DOI: 10.1093/dnares/dss005
关键词
pearl oyster; Pinctada fucata; draft genome
资金
- OIST Internal Fund for Marine Genomics Unit
- IT Section of the OIST
- Human Genome Center at the University of Tokyo
- Grants-in-Aid for Scientific Research [22228006, 23244101, 23340157] Funding Source: KAKEN
The study of the pearl oyster Pinctada fucata is key to increasing our understanding of the molecular mechanisms involved in pearl biosynthesis and biology of bivalve molluscs. We sequenced similar to 1150-Mb genome at similar to 40-fold coverage using the Roche 454 GS-FLX and Illumina GAllx sequencers. The sequences were assembled into contigs with N50 = 1.6 kb (total contig assembly reached to 1024 Mb) and scaffolds with N50 = 14.5 kb. The pearl oyster genome is AT-rich, with a GC content of 34%. DNA transposons, retrotransposons, and tandem repeat elements occupied 0.4, 1.5, and 7.9% of the genome, respectively (a total of 9.8%). Version 1.0 of the P. fucata draft genome contains 23 257 complete gene models, 70% of which are supported by the corresponding expressed sequence tags. The genes include those reported to have an association with bio-mineralization. Genes encoding transcription factors and signal transduction molecules are present in numbers comparable with genomes of other metazoans. Genome-wide molecular phylogeny su;4:ests that the lophotrochozoan represents a distinct clade from ecdysozoans. Our draft genome of the pearl oyster thus provides a platform for the identification of selection markers and genes for calcification, knowledge of which will be important in the pearl industry.
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