期刊
DNA REPAIR
卷 12, 期 9, 页码 691-698出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dnarep.2013.05.001
关键词
TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution
资金
- Wellcome International Senior Research Fellowship [WT076476]
- Czech Science Foundation [GACR 13-26629S, GACR P207/12/2323]
- European Regional Development Fund - (Project FNUSA-ICRC) [CZ.1.05/1.1.00/02.0123]
- European Social Fund
- state budget of the Czech Republic [CZ.1.07/2.3.00/20.0011]
- Hungarian Science Foundation [OTKA 101225, GOP-1.1.1-11-2011-0026, GOP-1.1.1-11-2012-0030]
- IPA Cross-border Co-operation Programme [HUSRB/1002/214/126]
Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes. (c) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
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