期刊
DNA REPAIR
卷 10, 期 2, 页码 138-148出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dnarep.2010.11.008
关键词
Meoisis; Homologous recombination; Resection; MRX complex; Double-strand break repair; DNA repair
资金
- BBSRC
- departmental doctoral training grant
- committee BBSRC
- MRC
- Krebs' Studentships
- Biotechnology and Biological Sciences Research Council [G20296] Funding Source: researchfish
During meiosis DNA double-strand breaks (DSBs) are induced and repaired by homologous recombination to create gene conversion and crossover products. Mostly these DSBs are made by Spo11, which covalently binds to the DSB ends. More rarely in Saccharomyces cerevisiae, other meiotic DSBs are formed by self-homing endonucleases such as VDE, which is site specific and does not covalently bind to the DSB ends. We have used experimentally located VDE-DSB sites to analyse an intermediate step in homologous recombination, resection of the single-strand ending 5' at the DSB site. Analysis of strains with different mutant alleles of MRE11 (mre11-58S and mre11-H125N) and deleted for EXO1 indicated that these two nucleases make significant contributions to repair of VDE-DSBs. Physical analysis of single-stranded repair intermediates indicates that efficient initiation and processivity of resection at VDE-DSBs require both Mre11 and Exo1, with loss of function for either protein causing severe delay in resection. We propose that these experiments model what happens at Spo11-DSBs after removal of the covalently bound protein, and that Mre11 and Exo1 are the major nucleases involved in creating resection tracts of widely varying lengths typical of meiotic recombination. (C) 2010 Elsevier B.V. All rights reserved.
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