期刊
DNA REPAIR
卷 9, 期 3, 页码 276-285出版社
ELSEVIER
DOI: 10.1016/j.dnarep.2009.12.016
关键词
AddAB; RecBCD; Dna2; Helicase-nuclease; Homologous recombination; Double-stranded DNA break repair
资金
- Royal Society
- Biotechnology and Biological Sciences Research Council
- European Research Council
Double-stranded DNA breaks are prepared for recombinational repair by nucleolytic digestion to form single-stranded DNA overhangs that are substrates for RecA/Rad51-mediated strand exchange. This processing can be achieved through the activities of multiple helicases and nucleases. In bacteria, the function is mainly provided by a stable multi-protein complex of which there are two structural classes; AddABand RecBCD-type enzymes. These helicase-nucleases are of special interest with respect to DNA helicase mechanism because they are exceptionally powerful DNA translocation motors, and because they serve as model systems for both single molecule studies and for understanding how DNA helicases can be coupled to other protein machinery. This review discusses recent developments in our understanding of the AddAB and RecBCD complexes, focussing on their distinctive strategies for processing DNA ends. We also discuss the extent to which bacterial DNA end resection mechanisms may parallel those used in eukaryotic cells. (C) 2010 Elsevier B.V. All rights reserved.
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