期刊
DNA REPAIR
卷 8, 期 7, 页码 834-843出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dnarep.2009.04.002
关键词
Base excision repair; Short-patch; Long-patch; BER fidelity
资金
- European Community [LSHG-CT-2005-512113]
- The Norwegian Cancer Society
- The Cancer Fund at St. Olavs Hospital
- The Svanhild and Arne Must Fund for Medical Research
- National Programme for Research in Functional Genomics in Norway (FUGE)
- Norwegian Research Council
Base excision repair (BER) of damaged or inappropriate bases in DNA has been reported to take place by single nucleotide insertion or through incorporation of several nucleotides, termed short-patch and long-patch repair, respectively. We found that extracts from proliferating and non-proliferating cells both had capacity for single- and two-nucleotide insertion BER activity. However, patch size longer than two nucleotides was only detected in extracts from proliferating cells. Relative to extracts from proliferating cells, extracts from non-proliferating cells had approximately two-fold higher concentration of POL beta, which contributed to most of two-nucleotide insertion BER. In contrast, two-nucleotide insertion in extracts from proliferating cells was not dependent on POL beta. BER fidelity was two- to three-fold lower in extracts from the non-proliferating compared with extracts of proliferating cells. Furthermore, although one-nucleotide deletion was the predominant type of repair error in both extracts, the pattern of repair errors was somewhat different. These results establish two-nucleotide patch BER as a distinct POL beta-dependent mechanism in non-proliferating cells and demonstrate that BER fidelity is lower in extracts from non-proliferating as compared with proliferating cells. (C) 2009 Elsevier B.V. All rights reserved.
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