期刊
DNA REPAIR
卷 7, 期 8, 页码 1192-1201出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dnarep.2008.03.020
关键词
hSNM1B; SNM1B; Apollo; DCLRE1B; SNM1; pso2; ARTEMIS; TRF2; telomere; ATM; ATM autophosphorylation; fanconi anemia; DNA-repair; yeast two hybrid; siRNA; p53; nuclear foci; DNA damage response
资金
- NCI NIH HHS [R01 CA057569, CA 57569, R01 CA057569-17] Funding Source: Medline
Human SNM1B/Apollc, is involved in the cellular response to DNA-damage, however, its precise role is unknown. Recent reports have implicated hSNM1B in the protection oftelomeres. We have found hSNMIB to interact with TRF2, a protein which functions in telomere protection and in an early response to ionizing radiation. Here we show that endogenous hSNM1B forms foci which colocalize at telomeres with TRF1 and TRF2. However, we observed that additional hSNM1B foci could be induced upon exposure to ionizing radiation (IR). In livecell-imaging experiments, hSNM1B localized to photo-induced double-strand breaks (DSBs) within 10 s post-induction. Further supporting a role for hSNM1B in the early stages of the cellular response to DSBs, we observed that autophosphorylation of ATM, as well as the phosphorylation of ATM target proteins in response to IR, was attenuated in cells depleted of hSNM1B. These observations suggest an important role for hSNM1B in the response to IR damage, a role that may be, in part, upstream of the central player in maintenance of genome integrity, ATM. (c) 2008 Elsevier B.V All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据