期刊
DNA AND CELL BIOLOGY
卷 31, 期 6, 页码 1095-1099出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/dna.2011.1559
关键词
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资金
- Natural Science Foundation [30860191]
- National Training Fund for the Basic Sciences of China [10730648]
- Major Projects for New Varieties of Genetically Modified Organisms, China [2008ZX08008-002]
The mammalian target of rapamycin (mTOR) is a Ser/Thr kinase. It plays an evolutionarily conserved role in regulating cell growth, proliferation, survival, and metabolism via different cellular processes. The purpose of this study was to explore the inhibitory effects of CCI-779 (temsirolimus), a specific mTOR inhibitor, on mTOR signaling, and examine the mechanism of cell growth suppression by CCI-779 in Cashmere goat fetal fibroblasts (GFb cells). GFb cells were sensitive to CCI-779 and the survival rate of cells treated with >3.0 mu M of CCI-779 was significantly reduced compared with the control (p < 0.01). CCI-779 inhibited the phosphorylation of mTOR (at Ser2448) and S6 (at Ser240/244), and the expression of mTOR, p70S6K, and S6. Thus, CCI-779 was toxic to GFb cells, and it induced a dose-dependent decrease in cell proliferation and caused G1/S cell cycle arrest. Taken together, these data show that CCI-779 can inhibit mTOR signaling and proliferation in GFb cells in vitro. Therefore, mTOR is an important regulator for GFb cell growth and proliferation.
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