Nucleic Acid Binding Behaviors and Cytotoxic Properties of a Ru(II) Complex

The interactions of complex [Ru(bpy)(2)(hnip)](2+) (1) {bpy 2,2'-bipyridine, hnip = 2-(2-hydroxy-1-naphthyl)imidazo [4,5-f][1,10]phenanthroline} with calf thymus DNA and yeast tRNA were investigated by UV-vis spectroscopy, fluorescence spectroscopy, viscosity, equilibrium dialysis, and circular dichroism. In addition, the antitumor activities of complex 1 were evaluated with MTT method. These results indicate that the structures of DNA and RNA have significant effects on the binding behaviors of complex 1. Further, complex 1 demonstrates different antitumor activities against selected cancer cell lines in vitro.