Simultaneous determination of insulin and its analogues in pharmaceutical formulations by micellar electrokinetic chromatography

A simple and efficient MEKC method was developed to simultaneously determine human insulin, its five analogues, the main degradation products and the excipients usually present in injection formulations. A very fast method with a total analysis time of 3 min was then successfully validated for the analysis of human insulin and the quality control of commercial formulations was carried out. (C) 2015 Elsevier B.V. All rights reserved.