期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 102, 期 -, 页码 276-281出版社
ELSEVIER
DOI: 10.1016/j.jpba.2014.09.027
关键词
Angiotensin-converting enzyme inhibitor; Bioactivity-guided fractionation; C-flavone glycosides; Desmodium styracifolium; High-speed counter-current chromatography
资金
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- New Century Excellent Talents in University [NCET-12-09-77]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1193]
A new strategy of the convergence of high-speed counter-current chromatography (HSCCC) and bioactive assay technique was developed for rapidly screening and separating the angiotensin-converting enzyme (ACE) inhibitors from the aerial parts of Desmodium styracifolium. Bioactivity-guided fractionation of the crude extract was first established to target the bioactive fractions based on HSCCC coupled with in vitro ACE inhibitory assay. Subsequently, the bioactive fractions were further separated by the recycling complexation HSCCC respectively, using 0.10 mol/L copper sulfate in the lower phase of two-phase solvent system composed of n-butanol/water (1:1, v/v). Five C-glycosylflavones, vicenin 2 (1), carlinoside (2), vicenin 1 (3), schaftoside (4) and vicenin 3 (5), were successfully obtained. Their chemical structures were identified using ESI-MS and NMR. All the isolates showed in vitro ACE inhibitory activity with the IC50 values between 33.62 and 58.37 mu M. The results demonstrated that the established method was proposed as an excellent strategy to systematically screen and purify active compounds from traditional Chinese medicines. (C) 2014 Elsevier B.V. All rights reserved.
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