期刊
DISEASES OF AQUATIC ORGANISMS
卷 106, 期 1, 页码 39-47出版社
INTER-RESEARCH
DOI: 10.3354/dao02617
关键词
Vibriosis; Vaccination; Recombinant plasmid; Outer membrane protein; pcDNA; Aquaculture
资金
- National Program on Key Basic Research Project [2009CB118704]
- 973 Program Earlier Research Project [2011CB111601]
- National Science Foundation of Guangdong Province [S2011010000257]
- Science and Technology Planning Project of Guangdong Province in the People's Republic of China [2009B050700040]
The outer membrane proteins of Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In the present study, the ompW gene was cloned, expressed and purified. A DNA vaccine was constructed by inserting the ompW gene into a pcDNA plasmid. Crimson snapper Lutjanus erythropterus (Bloch) were injected intramuscularly with the recombinant plasmid pcDNA-ompW. The expression of the DNA vaccine was detected in gill, head kidney, heart, liver, spleen and injection site muscle of crimson snapper by RT-PCR 7 and 28 d post-vaccination. The ELISA results demonstrated that the DNA vaccine produced an observable antibody response in all sera of the vaccinated fish. In addition, crimson snapper immunized with the DNA vaccine showed a relative percentage survival (RPS) of 92.53%, indicating effective protection against V. alginolyticus infection.
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