期刊
DIGESTIVE DISEASES AND SCIENCES
卷 58, 期 9, 页码 2623-2633出版社
SPRINGER
DOI: 10.1007/s10620-013-2690-1
关键词
Human esophageal cancer; TM4SF5; Integrin beta 1; Prognosis; Biomarker
资金
- National Natural Science Foundation of China [81260321, 309603381]
- National Natural Science Foundation of Hainan Province [811206]
Purpose We investigated expression of TM4SF5 and its involvement in human esophageal cancer (HEC). Methods We analyzed TM4SF5 expression in normal esophageal epithelial cells (HEEC), in four HEC cell lines, and in 20 HEC clinical tissue samples and matched nontumor samples. The effect of TM4SF5 on HEC cell proliferation and metastasis and invasion was assessed, and the relationship between TM4SF5 and integrin beta 1 was determined. Finally, TM4SF5 and integrin beta 1 expression were further examined by use of immunohistochemistry (IHC) and tissue microarray analysis, and the prognostic use of TM4SF5 and integrin beta 1 in HEC was evaluated. Results TM4SF5 was more highly expressed in HEC cells and in HEC tissues than in HEEC and matched nontumor tissues. Down-regulation of TM4SF5 in KYSE150 cells reduced cell proliferation and metastasis and invasion whereas metastasis and invasion by KYSE510 increased after TM4SF5 cDNA transfection. In HEC cells, TM4SF5 formed a complex with integrin beta 1, and interference with integrin beta 1 in KYSE510-TM4SF5 cells markedly inhibited cell invasion on laminin 5. Our findings also showed that TM4SF5 and integrin beta 1 overexpression correlated with low differentiation and high stage (p < 0.05, respectively). Postoperative 5-year overall survival of patients with TM4SF5(low) and/or integrin beta 1(low) was higher than for patients with TM4SF5(high) and/or integrin beta 1(high). Multivariate analysis demonstrated that TM4SF5 and integrin beta 1 co-overexpression was an independent prognostic marker for HEC. Conclusion TM4SF5 is positively associated with HEC invasiveness. The combination of TM4SF5 with integrin beta 1 could potentially serve as a novel marker for predicting HEC prognosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据