期刊
DIGESTIVE DISEASES AND SCIENCES
卷 54, 期 4, 页码 811-815出版社
SPRINGER
DOI: 10.1007/s10620-008-0434-4
关键词
Midkine; Pancreatic cancer; Quantitative real-time RT-PCR
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Fukuoka Cancer Society and Kaibara Morikazu Medical Science Promotion Foundation
Purpose Midkine (MK) has been reported to be a possible molecular marker for the diagnosis of pancreatic cancer. We investigated the feasibility of quantitative analysis of MK mRNA by quantitative real-time RT-PCR (qRT-PCR) as a promising tool for the diagnosis of pancreatic cancer. Results We found that pancreatic cancer tissues expressed significantly higher levels of MK mRNA than intraductal pancreatic mucinous neoplasm (IPMN) and non-neoplastic pancreatic tissues (P < 0.05); in contrast, we did not find any differences in MK mRNA expression between IPMN and non-neoplastic pancreatic tissues. Additionally, we observed that poorly differentiated carcinoma samples expressed higher levels of MK mRNA than well-differentiated carcinoma samples, although a significant difference was not observed. Conclusions The present data suggests that quantitative analysis of MK mRNA provides an objective and sensitive evaluation and may be a promising modality for the diagnosis of pancreatic cancer and the prediction of its prognosis.
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