期刊
DIFFERENTIATION
卷 85, 期 3, 页码 101-109出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.diff.2013.01.001
关键词
Human induced pluripotent stem cell; Milk teeth; Blood outgrowth endothelial cells; Tissue storage
资金
- Netherlands Institute of Regenerative Medicine (NIRM) [FES0908]
- FP7(Industem)
- Smart Mix Program of the Netherlands Ministry of Economic Affairs
- Netherlands Ministry of Education, Culture and Science
- Else-Kroener Fresenius grant
The generation of human induced pluripotent stem cells (hiPSCs) requires the collection of donor tissue, but clinical circumstances in which the interests of patients have highest priority may compromise the quality and availability of cells that are eventually used for reprogramming. Here we compared (i) skin biopsies stored in standard physiological salt solution for up to two weeks (ii) blood outgrowth endothelial cells (BOECs) isolated from fresh peripheral blood and (iii) children's milk teeth lost during normal replacement for their ability to form somatic cell cultures suitable for reprogramming to hiPSCs. We derived all hiPSC lines using the same reprogramming method (a conditional (FLPe) polycistronic lentivirus) and under similar conditions (same batch of virus, fetal calf serum and feeder cells). Skin fibroblasts could be reprogrammed robustly even after long-term biopsy storage. Generation of hiPSCs from juvenile dental pulp cells gave similar high efficiencies, but that of BOECs was lower. In terms of invasiveness of biopsy sampling, biopsy storage and reprogramming efficiencies skin fibroblasts appeared best for the generation of hiPSCs, but where non-invasive procedures are required (e.g. for children and minors) dental pulp cells from milk teeth represent a valuable alternative. (C) 2013 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.
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