期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 80, 期 3, 页码 185-190出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2014.08.006
关键词
Salmonella; Evaluation; Microarray; O-antigen; H-antigen; Serotyping
资金
- Genomics Research and Development Initiative through the Office of Biotechnology and Science, Health Canada
- Department for Environment, Food and Rural Affairs [OZ0333]
- Department for Innovation, University and Skills [DT0033]
Salmonella serotyping is an essential first step for identification of isolates associated with disease outbreaks. The Salmonella genoserotyping array (SGSA) is a microarray-based alternative to standard serotyping designed to rapidly identify 57 of the most commonly reported serovars through detection of the genes encoding surface 0 and H antigens and reporting the corresponding serovar in accordance with the existing White-Kaufmann-Le Minor serotyping scheme. In this study, we evaluated the SGSA at 4 laboratories in 3 countries by testing 1874 isolates from human and non-human sources. The SGSA correctly identified 96.7% of isolates from the target 57 serovars. For the prevalent and clinically important Salmonella serovars Enteritidis and Typhimurium, test specificity and sensitivity were greater than 98% and 99%, respectively. Due to its high-throughput nature, the SGSA is a rapid and cost-effective alternative to standard serotyping for identifying the most prevalent serovars of Salmonella. Crown Copyright (C) 2014 Published by Elsevier Inc. All rights reserved.
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