Antigen-specific CD4- and CD8-positive signatures in different phases of Mycobacterium tuberculosis infection

Current diagnostic standards for Mycobacterium tuberculosis (MTB) infection do not distinguish between active and latent tuberculosis (TB). To identify specific biomarkers characterizing the different forms of TB infection, we investigated in parallel with the QuantiFERON -TB Gold In-Tube (QFT-IT) the use of flow cytometry measuring CD4 and CD8 MTB-specific immune response in 17 active-TB patients, 21 health care workers (HCW), 14 recent contacts of TB patients (RC-TB), and 10 bacille Calmette Guerin (BCG)-vaccinated healthy controls (BCG-HC). A correlation (r = 0.4526, P = 0.0002) was found only between the amount of IFN-gamma measured by QFT-IT and the frequency of CD4+/CD69+/IFN-gamma+ T cells. The frequency of CD4+/CD69+/IFN gamma+ responding T cells was higher in active-TB patients (0.254 +/- 0.336%, P < 0.01) compared to the other groups. The response of QFT-IT antigen-specific CD8+/CD69+/IFN gamma+ T cells was significantly higher in RC-TB (0.245 +/- 0.305%, P < 0.05) compared to the other study groups. (C) 2013 Elsevier Inc. All rights reserved.