期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 75, 期 4, 页码 390-395出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2013.01.011
关键词
Leishmania; VL; PKDL; Diagnosis; LAMP
资金
- Indian Council of Medical Research (ICMR), India
Loop-mediated isothermal amplification (LAMP) is at the forefront in the search for innovative diagnostics for rapid and specific amplification of target DNA under isothermal conditions. We have applied LAMP assay using SYBR Green for clear-cut naked eye detection of Leishmania (Leishmania) donovani in 200 clinical samples of visceral leishmaniasis (VL) and post-kala-azar dermal leishmaniasis (PKDL). The assay was positive in 53/55 VL blood samples (sensitivity, 96.4%; 95% confidence interval [CI], 87.7-99%), 15/15 VL bone marrow aspirate samples (sensitivity, 100%; 95% CI, 79.6-100%), 60/62 PKDL tissue biopsy samples (sensitivity, 96.8%; 95% CI, 88.9-99.1%), and 1/68 control samples (specificity, 98.5%; 95% CI, 92.1-99.7%). The assay was specific for L (L) donovani, the causative species for VL and negative for L (L) infantum, L (L) tropica, and L (L) major. This is the first comprehensive clinical study demonstrating the applicability of the LAMP assay for a rapid and reliable molecular diagnosis of VL and PKDL. (C) 2013 Elsevier Inc. All rights reserved.
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