4.3 Article

Triplex PCR assay for the rapid identification of 3 major Vibrio species, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis

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出版社

ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2013.04.005

关键词

Haemolysin gene; Diagnostics; Epidemiology; Haem-utilising gene; Toxin-regulatory gene

资金

  1. Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India [BT/PR/11634/INF/22/104/2008]
  2. Puri Foundation for Education in India
  3. DBT

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A triplex PCR assay was developed for the identification of 3 major Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis by targeting their haemolysin, haem-utilizing, and central regulatory genes, respectively. This simple, rapid, sensitive, and specific assay using cell lysates from 227 samples established its usefulness in research and epidemiology. (c) 2013 Elsevier Inc. All rights reserved.

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