期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 72, 期 1, 页码 52-61出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2011.09.010
关键词
Drug resistant; Korea; Mutation analysis; Mycobacterium tuberculosis; Tuberculosis
资金
- Korea Science and Engineering Foundation (KOSEF)
- Korean government (MEST) [R01-2008-000-12139-0]
- National Research Foundation of Korea [R01-2008-000-12139-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We investigated the causal relationship between genotype and phenotype of drug-resistant Mycobacterium tuberculosis isolates obtained from patients with pulmonary tuberculosis (TB) in Korea. Of 80 isolates tested, 17, 20, 1, and 7 isolates were mono-resistant to ethambutol (EMB), isoniazid (INH), pyrazinamide (PZA), and rifampicin (RFP), respectively, and 31 isolates (38.8%) were multidrug-resistant (MDR). Sequencing analysis showed that 78% (32/41) of RFP-resistant strains had mutations in the rifampicin resistance determining region (RRDR) of rpoB, and the mutation at rpoB531 (59.4%) was most abundant. In 52 INH-resistant strains, mutations were found mostly at C-15T (n = 21, 40.4%) in the inhA promoter region as well as at katG315 (n = 12, 23.1%). Mutations at embB306 were mostly found in 26.7% (12/45) of EMB-resistant isolates. New mutations found here in MDR isolates include rpoB523 (Gly523Glu) and embB319 (Tyr319Ser). Consequently, mutations in the rpoB531, C-15T in the inhA promoter region, embB306, and katG315 would be a useful marker for rapid detection of MDR M. tuberculosis isolates in Korea. (C) 2012 Elsevier Inc. All rights reserved.
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