期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 71, 期 2, 页码 144-150出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2011.06.021
关键词
Extrapulmonary tuberculosis; Biomarker; Decision tree model; Weak cationic exchange magnetic beads; MALDI-TOF MS
资金
- Ministry of Health, China [2008ZX10003-012]
Matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS) combined with weak cationic exchange (WCX) magnetic beads was used to establish a decision tree model that distinguished extrapulmonary tuberculosis (EPTB) from non-EPTB individuals. Eight-one patients with EPTB and 112 non-EPTB individuals (72 disease controls and 40 healthy controls) were involved in this study. The model was set up by 5 of 19 differentially expressed peaks (P < 0.05), m/z 4100, 4310, 6093, 8605, and 14,019. This model can discriminate patients with EPTB from non-EPTB with a sensitivity of 97.7% and a specificity of 84.1%. The test set verified that this model had good sensitivity and specificity: 94.4% and 83.6%, respectively. In conclusion, MALDI-TOF MS combined with WCX magnetic beads is a powerful technology for constructing a decision tree model and the model we built could serve as a potential diagnostic tool for EPTB. (C) 2011 Elsevier Inc. All rights reserved.
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