期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 66, 期 1, 页码 41-49出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2008.07.011
关键词
Tag polymerase activity; DNA-free PCR reagent; Universal 16S rDNA PCR; Bacteremia; Sensitivity of detection in blood
资金
- German Ministry of Economy and Labor
Universal 16S rRNA gene polymerase chain reaction (PCR) is a promising means of detecting bacteremia. Among other factors, the PCR reagents play a prominent role for obtaining a high sensitivity of detection. The reagents are ideally optimized with respect to the amplifying activity and absence of contaminating DNA. In this study, it was shown in a universal 16S rDNA real-time PCR assay that commercial PCR reagents can vary greatly among each other in these characters. Only 1 of the 5 reagents tested met the criteria of sensitive detection of pathogen DNA with a minimum of false-positive results. The reagent was validated by the detection of pathogens at low titers using bacterial DNA extracted from blood that was spiked with various Gram-positive and Gram-negative bacteria. (C) 2010 Elsevier Inc. All rights reserved.
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