期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 68, 期 2, 页码 152-158出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2010.06.011
关键词
Leishmaniasis; Species identification; PCR-RFLP
资金
- EMRO/TDR [SGS05/79]
Topoisomerase II gene of Leishmania genus was used to develop a molecular tool for detection and species differentiation of Leishmania from clinical samples. Identification was achieved by a polymerase chain reaction followed by digestion with 2 restriction endonucleases BstU1 and Taq1. Despite the relatively low sensitivity, it is able to differentiate between 3 complexes responsible for cutaneous leishmaniasis. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
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