期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 66, 期 2, 页码 175-180出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2009.08.013
关键词
Cutaneous leishmaniasis; Leishmania; Diagnosis; Species identification; PCR; RFLP; French Guiana
资金
- University of the French West Indies and French Guiana
- French Ministere de l'Enseignement Superieur et de la Recherche Scientifique
Leishmania (Viannia) guyanensis was for many years the only species commonly identified in French Guiana, but precise species identifications were quite rare. We describe a new restriction fragment length polymorphism polymerase chain reaction technique using a 615-bp fragment of the RNA polymerase II gene and 2 restriction enzymes, TspRI and HgaI. Seven reference strains (Leishmania (Leishmania) amazonensis, Leishmania (Viannia) lainsoni, Leishmania (Viannia) braziliensis, L. (V.) guyanensis, Leishmania (Viannia) naiffi, Leishmania (Leishmania) major, Leishmania (Leishmania) infantum) and 112 clinical samples from positive lesions were used for the development of the technique. The rates of positive species identification were 85.7% for punch skin biopsy specimens, 93.1% for positive Giemsa-stained smears, and 100% for positive culture supernatants. In the framework of cutaneous leishmaniasis species surveillance for the 2006 to 2008 period, parasite identification was carried out for 199 samples from different patients. The prevalence of the various Leishmania spp. was 84.4% for L. (V.) guyanensis, 8.0% for L. (V) braziliensis, 5.0% for L. (L.) amazonensis, and 2.6% for L. (V.) lainsoni. L. (V) braziliensis seems to be locally an emerging pathogen. (C) 2010 Elsevier Inc. All rights reserved.
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