期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 65, 期 3, 页码 327-330出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2009.07.003
关键词
Salmonella enterica; serotyping; multiplex PCR
To improve limitations of Salmonella serotyping, 2 multiplex polymerase chain reaction (M-PCR) were developed using a strategy that identifies first the genes encoding serogroups (rfbJ, wzx). According to the serogroup determined, a second M-PCR identifies serotype (fliC,,fljB, wcdB, and sdf-I sequence). Standardization and evaluation of both M-PCRs were carried out. (C) 2009 Elsevier Inc. All rights reserved.
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